Flow sorting from organ material by intracellular markers

Flow sorting from organ material by intracellular markers.

BACKGROUND Fluorescence-activated cell sorting (FACS) is an attractive technique for gene or protein expression studies in rare cell populations. For cell types where specific surface markers are not known, intracellular markers can be used. However, this approach is currently held to be difficult, as the required fixation and permeabilization may cause protein modification and RNA degradation....

Cardiac material markers from tagged MR images

Tagged magnetic resonance imaging (MRI) has shown great promise in non-invasive analysis of heart motion. To replace implanted markers as a gold standard, however, tagged MRI must be able to track a sparse set of material points, so-called material markers, with high accuracy. This paper presents a new method for generating accurate motion estimates over a sparse set of material points using st...

Chromosome measurement and sorting by flow systems.

A flow microfluorometer was used to measure metaphase chromosomes in suspension at rates up to 100,000 per min. Chromosomes from cells of the Chinese hamster M3-1 cell line were isolated, stained for DNA with the fluorescent dye ethidium bromide, and analyzed for DNA content. Nine distinct peaks were resolved that correspond well with independent chromosomal DNA measurements made with a high-re...

Protein sorting to intracellular compartments

The organization of eukaryotic cells depends on correct targeting of proteins and lipids to the organelles at which they function, but how organelle identity is maintained in the face of a constant flux of material is a major problem for the cell. New mechanisms addressing this question were revealed at the Minisymposium on " Membrane Traffic: Dynamic and Regulation. " Starting with the endopla...

Phosphoproteins by Flow Cytometry Surface Markers and Intracellular Coordinate Analysis of Murine Immune Cell